Invitrogen™ Recombinant Protein G Agarose

• Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol)
• The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads
• rProtein G Agarose has a binding capacity of approximately 20mg of human IgG permL of beads
• rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A
• The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography
• To purify IgG, load crude samples (serum, ascites fluid, or hybridoma supernatant) onto a column of rProtein G Agarose under physiologic conditions of salt and pH
• Non-IgG components do not bind to the beads and elute in the flow through fraction; Purified IgG is then eluted with a low pH buffer

PCR, PCR and Real-Time PCR