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Invitrogen™ IR beta Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ KHR9111
Additional Details : Weight : 0.54000kg
Includes: IR Antibody-Coated 96-Well Plate; IR (β-subunit) Standard; Standard Diluent Buffer; IR (β-subunit) Detection Antibody; anti-rabbit IgG-HRP (100X); HRP Diluent; Wash Buffer Concentrate (25X); Stabilized Chromogen, TMB; Stop Solution; Plate Covers; Detailed protocol with validation tests
Description
The Human Insulin Receptor beta subunit (Hu IRβ) ELISA quantitates Hu IRβ in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu IRβ. Cross-reactivity has been observed in mouse and rat cells. Principle of the method The Human IRβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The insulin receptor (IR) is a heterodimeric protein complex that has an intracellular beta subunit and an extracellular alpha subunit, which is disulfide- linked to a transmembrane segment. The insulin ligand binds to the IR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin.Order Info
Shipping condition: Wet ice
Specifications
P06213,P15127,P15208 | |
0.5 ng/mL | |
HRP | |
Cell Lysates | |
Human, Mouse, Rat | |
16337, 24954, 3643 | |
4 hr. | |
5.4% | |
HRP | |
96 Tests | |
Cell Lysate,10 μL | |
Human, Mouse, Rat | |
4 hr. |
0.94-60 ng/mL | |
0.94 to 60ng/mL | |
ELISA Kit | |
Receptor Tyrosine Kinases | |
Colorimetric Microplate Reader | |
CD220,HHF5 | |
8.2% | |
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
IR | |
RUO | |
2°C to 8°C | |
1 hr. 20 min. |
For Research Use Only. Not for use in diagnostic procedures.