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Invitrogen™ TNF alpha Human ELISA Kit, Ultrasensitive

Quantifies natural and recombinant human Tumor Necrosis Factor Alpha (TNF-α) in serum, plasma, buffered solution, or cell culture medium

7480.00 SEK - 18470.00 SEK

Specifications

Accession Number P01375
Assay Range 0.5-32 pg/mL
Assay Sensitivity <0.09 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

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10465133
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Invitrogen™
KHC3014
96 Tests
7480.00 SEK
96 tests
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10160573
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Invitrogen™
KHC3013
2 x 96 Tests
13760.00 SEK
192 tests
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Invitrogen™
KHC3014C
5 x 96 Tests
18470.00 SEK
Pack of 5
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Estimated Shipment: 16-04-2024
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Description

Description

The Human Tumor Necrosis Factor Alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFα. Principle of the method The Human TNFα ultrasensitive solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF-α is produced by macrophages and has many immune effects. Ultrasensitive ELISA Kits are approximately 10X more sensitive than the conventional ELISA Kit.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

P01375
<0.09 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
5.9%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
0.5-32 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric
7124
8.5%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
<0.09pg/mL
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Human
3 hr.
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.