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Invitrogen™ TNF alpha Porcine ELISA Kit

Quantifies natural and recombinant swine TNF-α in swine serum, EDTA plasma, buffered solution, or cell culture medium

6220.00 SEK - 8870.00 SEK

Specifications

Accession Number P23563
Assay Range 23.4-1500 pg/mL
Assay Sensitivity <3 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

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10435133
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Invitrogen™
KSC3011
96 Tests
6220.00 SEK
Each
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10069402
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Invitrogen™
KSC3012
2 x 96 Tests
8870.00 SEK
Each
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Description

Description

The Porcine Tumor Necrosis Factor alpha (Por TNFα) ELISA quantitates Por TNFα in porcine serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Por TNFα1. Principle of the method The Pociner TNFαsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF-α is produced by macrophages and has many immune effects.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Wet Ice

Specifications

Specifications

P23563
<3 pg/mL
ELISA Kit
Porcine
Colorimetric Microplate Reader
TNFa
7.3%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
TNF-α
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Porcine
5 hr.
23.4-1500 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric Detection
397086
4 hr.
6%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
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Certificates

For Research Use Only. Not for use in diagnostic procedures.