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Macherey-Nagel™ NucleoSpin™ DNA Stool
Combines enzymatic lysis with mechanical disruption of cell walls for efficient isolation of high-quality genomic DNA from any stool sample, fresh or frozen. Complete kit includes NucleoSpin™ Bead Tubes.
Brand: Macherey-Nagel™ 740472.10
Additional Details : Weight : 0.25000kg Transport : UN number : 1993 Chem class : 3 Pack group : III
Description
Isolation of genomic DNA from bacterial and epithelial cells from stool samples
- Efficient procedure combining enzymatic lysis and bead disruption
- Isolation of high-quality genomic DNA (microbial and host) from fresh and frozen samples
- Suitable for any stool sample – carnivores, omnivores, and herbivore
- Bead Tubes included for efficient lysis – compatible with most common disruption devices
- NucleoSpin™ Inhibitor Removal Columns ensure highest purity of the isolated DNA
- Downstream applications: PCR, real-time PCR, Southern blotting, microarray technology
Specifications
| Room temperature (18 - 25 °C) | |
| Silica MembraneTechnology and Mechanical (Bead Tube) Disruption | |
| Binding capacity: 50 μg | |
| Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer | |
| DNA Stool Columns, Bead Tubes Type A, Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), Buffers | |
| A260/A280: 1.7 - 1.9 | |
| Fragment size: 200 bp - approx. 50 kbp | |
| Varies by sample type, quality and water content |
| Mini Spin Column | |
| Stool Samples (Herbivores, Omnivores, or Carnivores, Fresh or Frozen) | |
| Elution volume: 30 - 100 μL | |
| Isolation of genomic DNA from stool samples | |
| 10 | |
| Approx. 200 mg (180 - 220 mg) | |
| 60 min/10 prep |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
- Warning
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
- H412-Harmful to aquatic life with long lasting effects.