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Macherey-Nagel™ Nucleospin™ Plasmid Transfection-grade
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.
1150.00 SEK - 5055.00 SEK
Specifications
Bacterial Endotoxins | <50 EU/ng DNA |
---|---|
Content And Storage | Room temperature (18 - 25 °C) |
Format | Mini Spin Column |
Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
Product Code | Brand | No. of Samples | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | No. of Samples | Price | Quantity & Availability | |||||
15503636
|
Macherey-Nagel™
740490.50 |
50 |
1150.00 SEK
50 reactions |
Estimated Shipment: 04-07-2024 Log in to see stock. |
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15593626
|
Macherey-Nagel™
740490.250 |
250 |
5055.00 SEK
250 reactions |
Estimated Shipment: 04-07-2024 Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via manual, vacuum or centrifugation processing
- Ultra-fast procedure using mini spin columns simplifies the work-flow
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications
<50 EU/ng DNA | |
Mini Spin Column | |
Bacterial Culture (E. coli, High-copy Plasmids) | |
Elution Volume: 30 - 50 μL | |
Centrifuge, Vacuum manifold (e.g. NucleoVac 96) | |
NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A | |
Up to 5 mL | |
14 min/6 preps |
Room temperature (18 - 25 °C) | |
Silica Membrane and Endotoxin-reduction Technology | |
Binding capacity: 35 μg | |
Transfection-grade plasmid DNA | |
Rapid purification of plasmids for transfection | |
A260/A280: 1.80 - 1.85 | |
< 15 kbp | |
15 - 30 μg |