OCT3/4, clone: EM92, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5841-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).Applications Reported: This EM92 antibody has been reported for use in immunoblotting (WB).Applications Tested: This EM92 antibody has been tested by western blot analysis of F9 and P19 embryonal carcinoma cell lysates. For western blotting, this antibody can be used at a starting dilution 2 ug/mL. However, the antibody should be titrated for individual experiments.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Octamer-binding transcription factor 3 antibody, Oct-3, OCT3, POU domain class 5 transcription factor 1 antibody, Oct-4, OTF3 antibody.|
|Immunohistochemistry, Western Blot|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.