OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

5'...C^T N A G...3'
3'...G A N T^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
• Incubate at 37°C
• Enzyme Activity in OPTIZYME buffers:
• Buffer 1: 20 - 50%
• Buffer 2: 20 - 50%
• Buffer 3: 20 - 50%
• Buffer 4: 100%
• Buffer 5: 20 - 50%

Storage Buffer:
• 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
l
• Ligation and Re-cleavage:
• More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.
• Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I
• Methylation Effects:
• Dam: Never overlaps - no effect
• Dcm: Never overlaps - no effect
• CpG: Never overlaps - no effect
• EcoKI: Never overlaps - no effect
• EcoBI: May overlap - effect not determined