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OPTIZYME™ Sau3AI (Bsp143I), Fisher BioReagents™

Produktkod. 11954792
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Kvantitet:
300 U
Förpackningsstorlek:
Varje
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Produktkod. 11954792

Brand: Fisher BioReagents BP80301

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Beskrivning

5'...^G A T C ...3'
3'... C T A G^...5'

Supplied with: 10X OPTIZYME Buffer Sau3AI
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer Sau3AI: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate, 0.02% Triton X-100 and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 10mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with Sau3AI
  • Compatible Ends: BamHI, BclI, BglII, PsuI
  • Methylation Effects:
  • Dam: Completely overlaps - no effect
    • 5'...Gm6ATC...3'
  • (No effect on cleavage)
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - blocked
    • 5'...GATm5C G...3'
  • 3'...CTA Gm5C...5'
  • (Cleavage is blocked)
  • EcoKI: Never overlaps - no effect
    • 5'...TGm6ATC(N)6 TGCT...3'
  • 3'...AC TAG(N)6 m6ACGA...5'
  • (No effect on cleavage)
  • EcoBI: May overlap - no effect

    • TRUSTED_SUSTAINABILITY

    Specifikationer

    Koncentration 10 U/μL
    Komponenter 10X OPTIZYME™ Buffer Sau3AI
    Inkubatortemperatur 37°C
    pH 7.4
    För användning med (applikation) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Sau3AI
    Förvaringsbuffert 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    Kvantitet 300 U
    Klipp webbplats .GATC.
    Produkttyp Sau3AI
    Form Liquid
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