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  5. 11914802

OPTIZYME™ ScaI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8037-1

Additional Details : Weight : 0.01000kg

Product Code. 11914802

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Description

Description

5'...A G T^A C T...3'
3'...T C A^T G A...5'

Supplied with: 10X OPTIZYME AarI, ScaI, PasI Buffer
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer AarI, ScaI, PasI: 10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 0 - 20%
  • Buffer 3: 0 - 20%
  • Buffer 4: 0 - 20%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 80% of the DNA fragments can be ligated and more than 90% of these can be re-cut after 50-fold over-digestion with ScaI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 20 units of ScaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Notes:
    • Conditions of low salt, high enzyme concentration, glycerol concentration >5% or pH >8.0 may result in star activity.
    • Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.
    Methylation Effects:
    Dam: Never overlaps - no effect
    Dcm: Never overlaps - no effect
    CpG: Never overlaps - no effect
    EcoKI: Never overlaps - no effect
    EcoBI: May overlap - blocked
    • 5'...TGm6AGTACT(N)3 TGCT...3'
  • 3'...AC TCATGA(N)3 m6ACGA...5'
  • (Cleavage blocked)

    • Specifications

    Specifications

    10 U/μL
    37°C
    >80% of DNA fragments can be ligated and >90% of these can be re-cut after 50-fold over-digestion with ScaI
    1000 U
    ScaI
    10X OPTIZYME™ AarI, ScaI, PasI Buffer
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    AGT.ACT
    Liquid
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