OVA257-264 (SIINFEKL) peptide bound to H-2Kb, clone: eBio25-D1.16 (25-D1.16), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5743-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Cells can be pulsed with the SIINFEKL peptide according to the following protocol:1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.2. Incubate cells at 37°C for 2 hours.3. Wash cells with flow staining buffer.4. Proceed with cell surface staining as normal.For additional information see the references listed below.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|OVA257-264 (SIINFEKL) peptide bound to H-2Kb|
|PBS with 0.09% sodium azide; pH 7.2|
|H-2Kb-SIINFEKL, OVA-Kb, OVA257-264 H-2Kb, OVA257-264|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
|Flow Cytometry, Immunohistochemistry|
For Research Use Only.