DiscoverX PathHunter™ eXpress CCR4 Total GPCR Internalization Assay

The PathHunter™ CCR4 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit assesses ligand (e.g. CCL22/MDC) based internalization of CCR4 via detection of localization to the early endosome. This product uses EFC technology. The included cells overexpress PK-tagged CCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.