DiscoverX PathHunter™ eXpress EDNRB Total GPCR Internalization Assay

The PathHunter™ EDNRB Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit assesses ligand (e.g. endothelin-3) based internalization of EDNRB via detection of localization to the early endosome. This product uses EFC technology. The included cells overexpress PK-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.