DiscoverX PathHunter™ eXpress PTGIR Total GPCR Internalization Assay

The PathHunter™ PTGIR Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit assesses ligand (e.g. prostaglandin I2) based internalization of PTGIR via detection of localization to the early endosome. This product uses EFC technology. The included cells overexpress PK-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.