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Perforin Rat anti-Mouse, PE, Clone: eBioOMAK-D, eBioscience™

Rat Monoclonal Antibody

1100.00 SEK - 2805.00 SEK

Specifications

Antigen Perforin
Clone eBioOMAK-D
Target Species Mouse
Conjugate PE
Isotype IgG2a, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15299349 View Documents Affymetrix eBioscience
12-9392-80
25 μg 1100.00 SEK
25µg View alternative products
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EU Stock 3
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15209359 View Documents Affymetrix eBioscience
12-9392-82
100 μg 2805.00 SEK
100µg View alternative products
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EU Stock 5
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Description

Description

In contrast stimulation of splenocytes with anti-CD3/CD28 antibodies does result in an increase of perforin on both NK cells and CD8 cells. eBioOMAK-D is also crossreactive to human perforin and co-stains CD56 positive cells in PBMC. Expression of perforin and Granzyme B do not always correlate (as discussed above in the CD8 population of IL-2 stimulated splenocytes). Granzyme B typically is expressed earlier and at higher levels. Expression of Granzyme B is dramatically increased (more than 10,00 fold based on mRNA estimates and significantly at the protein level based on western blotting and flow analysis) compared to a minimal increase (10-100 fold) in perforin mRNA and protein with IL-2 stimulation. For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse perforin, it is highly recommended to use the Foxp3 buffer system (cat. 00-5523). Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com. Applications Reported: This eBioOMAK-D antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBioOMAK-D antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for intracellular (nuclear) proteins. This can be used at less than orequal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
Specifications

Specifications

Perforin
Mouse
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
eBioOMAK-D
PE
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.