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Phospho-BTK/ITK (Tyr551, Tyr511) Mouse anti-Human, Mouse, Clone: M4G3LN, eBioscience™

Mouse Monoclonal Antibody

1378.00 SEK - 3215.00 SEK

Specifications

Antigen Phospho-BTK/ITK (Tyr551, Tyr511)
Clone M4G3LN
Target Species Human, Mouse
Conjugate Unconjugated
Isotype IgG2b, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15547566
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Affymetrix eBioscience
14-9015-80
25 μg
1378.00 SEK
25µg
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Estimated Shipment
02-03-2021
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15557566
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Affymetrix eBioscience
14-9015-82
100 μg
3215.00 SEK
100µg
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Estimated Shipment
02-03-2021
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Description

Description

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton′s tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency. Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting. Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

(Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.). Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 µg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
Specifications

Specifications

Phospho-BTK/ITK (Tyr551, Tyr511)
Human, Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C
M4G3LN
Unconjugated
Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.