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Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen 44680G

Additional Details : Weight : 0.50000kg

Product Code. 10424503

  • 4040.00 SEK / Each
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For Research Use Only. All usage must comply with product instructions.


Description

Description

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK1&2. The final product is generated by affinity chromatography using an ERK1&2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop. This antibody has been used in western blotting and previous lots have been used in immunostaining. Other applications may work but have not been tested. The positive control used in western analysis were PC12 cells +/- Sorbitol; NIH3T3 +/- PDGF; and HEK293 +/- UV.

Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal antibody specifically detects Phospho-ERK1/ERK2 (Thr185, Tyr187) in Bovine, C. elegans, Chicken, Drosophila, Human, Mouse, Rat, Xenopus laevis samples. It is validated for Immunohistochemistry (Paraffin), Western Blot.
Specifications

Specifications

Phospho-ERK1/ERK2 (Thr185, Tyr187)
Polyclonal
Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
P21708, P26696, P27361, P28482, P40417, P46196, P63085, P63086, Q63844
MAPK1, mapk1.S, MAPK3, rl
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
100 μL
Primary
Bovine, C. elegans, Chicken, Drosophila, Human, Mouse, Rat, X. laevis
Antibody
IgG
Immunohistochemistry (Paraffin), Western Blot
Unconjugated
MAPK3
12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; CG18732; CT34260; CT39192; DERK; D-ERK; DERK-A; Diphospho-ERK; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERKA; DmERK-A; DmMAPK; dpERK; dp-ERK; dpERK1; dpMAPK; drosophila ERK; Dsor2; E(sina)7; EC2-1; EK2-1; enhancer of seven in absentia 7; ERK; Erk MAP kinase; Erk/Map kinase; ERK/rolled; Erk1; Erk-1; erk1 erk2; Erk1/2; ERK1b; ERK2; ERK-2; ERKA; ERK-A; ERT1; ERT2; Esrk1; extracellular signal-regulated kinase; extracellular signal-regulated kinase (ERK2); extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; EY2-2; fi06b09; GroupI
Rabbit
Antigen affinity chromatography
RUO
116590, 26413, 26417, 327672, 3354888, 373953, 398985, 50689, 531391, 5594, 5595
-20°C
Liquid
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