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Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Brand: Invitrogen 44680G
Additional Details : Weight : 0.50000kg
Description
Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK1&2. The final product is generated by affinity chromatography using an ERK1&2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop. This antibody has been used in western blotting and previous lots have been used in immunostaining. Other applications may work but have not been tested. The positive control used in western analysis were PC12 cells +/- Sorbitol; NIH3T3 +/- PDGF; and HEK293 +/- UV.
Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal antibody specifically detects Phospho-ERK1/ERK2 (Thr185, Tyr187) in Bovine, C. elegans, Chicken, Drosophila, Human, Mouse, Rat, Xenopus laevis samples. It is validated for Immunohistochemistry (Paraffin), Western Blot.Specifications
| Phospho-ERK1/ERK2 (Thr185, Tyr187) | |
| Polyclonal | |
| Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3 | |
| P21708, P26696, P27361, P28482, P40417, P46196, P63085, P63086, Q63844 | |
| MAPK1, mapk1.S, MAPK3, rl | |
| The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly. | |
| 100 μL | |
| Primary | |
| Bovine, C. elegans, Chicken, Drosophila, Human, Mouse, Rat, X. laevis | |
| Antibody | |
| IgG |
| Immunohistochemistry (Paraffin), Western Blot | |
| Unconjugated | |
| MAPK3 | |
| 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; CG18732; CT34260; CT39192; DERK; D-ERK; DERK-A; Diphospho-ERK; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERKA; DmERK-A; DmMAPK; dpERK; dp-ERK; dpERK1; dpMAPK; drosophila ERK; Dsor2; E(sina)7; EC2-1; EK2-1; enhancer of seven in absentia 7; ERK; Erk MAP kinase; Erk/Map kinase; ERK/rolled; Erk1; Erk-1; erk1 erk2; Erk1/2; ERK1b; ERK2; ERK-2; ERKA; ERK-A; ERT1; ERT2; Esrk1; extracellular signal-regulated kinase; extracellular signal-regulated kinase (ERK2); extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; EY2-2; fi06b09; GroupI | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 116590, 26413, 26417, 327672, 3354888, 373953, 398985, 50689, 531391, 5594, 5595 | |
| -20°C | |
| Liquid |