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Phospho-Histone H3 (Ser28) Monoclonal Antibody (HTA28), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9124-42

2290.11 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.23750kg

Product Code. 15510087

  • 3435.00 SEK / 100 tests
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Description

Description

Description: The HTA28 monoclonal antibody recognizes phosphorylated serine 28 of human, mouse, rat, bovine, and hamster histone H3. This 15 kDa protein is a component of eukaryotic chromatin that is involved in forming the nucleosome structure. Histone H3 can be phosphorylated at serine 10 and serine 28. Studies have demonstrated that phosphorylation at serine 28 is mediated by MSK1 following activation by the MAP kinase signaling pathway in response to tumor promoters (e.g., UV and EGF) and oncoproteins (e.g., c-Myc, c-Jun, and c-Fos). Histone H3 serine 28 phosphorylation has been linked to chromosome condensation during mitosis, cell transformation, and regulation of RNA polymerase III transcription machinery. Applications Reported: This HTA28 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This HTA28 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of nocodazole-treated HeLa cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Protocols: We recommend using Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins or Protocol C: Two-step protocol: Fixation/Methanol.

Phospho-Histone H3 (Ser28) Monoclonal antibody specifically detects Phospho-Histone H3 (Ser28) in Human, Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

Phospho-Histone H3 (Ser28)
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P68431, P68433, P84228, Q71DI3
H3C1, H3C10, H3C12, H3C14, H3C15, H3C2, H3C7, HIST1H3A
Affinity chromatography
RUO
126961, 260423, 319150, 319152, 333932, 360198, 8350, 8356, 8357, 8358, 8968, 97114
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
HTA28
eFluor 660
H3C10
BUR5; CG31613; CG31613-PA; CG33803; CG33806; CG33809; CG33812; CG33815; CG33818; CG33821; CG33824; CG33827; CG33830; CG33833; CG33836; CG33839; CG33842; CG33845; CG33848; CG33851; CG33854; CG33857; CG33860; CG33863; CG33866; CG5825; CG5825-PA; CG5825-PC; CG5825-PD; CG8989; dH3.3A; Dmel\CG31613; Dmel\CG5825; Dmel_CG31613; Dmel_CG5825; fb58e10; h3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3B.1; H3 histone, family 3C; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3.3; H3.3 histone A; H3.3 histone B; H3.3a; H3.3B; H3.5; H3.5 histone; H3.A; H3.B; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3B; H3-5; H3-53; H3-614; H3a; H3b; H3-B; H3C1; H3c10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6
Rat
100 Tests
Primary
Human, Mouse
Antibody
IgG2a κ
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