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Phospho-Syk (Tyr348) Monoclonal Antibody (moch1ct), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

1436.00 SEK - 3435.00 SEK

Specifications

Antigen Phospho-Syk (Tyr348)
Clone moch1ct
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15568216
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Affymetrix eBioscience
17-9014-41
25 Tests
1436.00 SEK
25 tests
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Estimated Shipment: 10-04-2024
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15578216
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Affymetrix eBioscience
17-9014-42
100 Tests
3435.00 SEK
100 tests
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Description

Description

Description: This moch1ct monoclonal antibody recognizes human and mouse spleen tyrosine kinase (also known as SYK) when phosphorylated on tyrosine 348 (Y348). SYK is the founding member of the SYK family of kinases that also includes ZAP-70 (zeta-associated protein of 70 kD) and is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. SYK is critical for B cell receptor (BCR) signaling and B cell development. Autophosphorylation of at Y348 is necessary for SYK to become fully catalytically active and creates a docking site for the SH2 domains of Vav, Grb2, p85 subunit of PI3 kinase, and PLC gamma. Specificity of this moch1ct clone was confirmed by ELISA, flow cytometry, and western blotting. Applications Reported: This moch1ct antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This moch1ct antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Phospho-Syk (Tyr348) Monoclonal antibody specifically detects Phospho-Syk (Tyr348) in Human, Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

Phospho-Syk (Tyr348)
5 μL/Test
Monoclonal
Liquid
RUO
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72Syk; p72-Syk; ptk72; SP1; spleen associated tyrosine kinase; spleen tyrosine kinase; SYK; syk signaling; Sykb; tyrosine-kinase syk; tyrosine-protein kinase SYK
Syk
Primary
4° C, store in dark, DO NOT FREEZE!
Syk
moch1ct
Flow Cytometry
APC
Mouse
Human, Mouse
P43405, P48025
20963, 6850
IgG1 κ
Affinity chromatography
Antibody
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