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Phospholamban Monoclonal Antibody (2D12), Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen MA3922
Additional Details : Weight : 0.01000kg
Description
MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse. MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues. The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.
Phospholamban Monoclonal antibody specifically detects Phospholamban in Canine, Chicken, Guinea Pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat samples. It is validated for Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot.Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
Specifications
Phospholamban | |
Monoclonal | |
1 mg/mL | |
PBS with 1mg/mL BSA and 0.05% sodium azide | |
P26677, P26678, P61012, P61013, P61014, P61015, P61016 | |
PLN | |
Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine Phospholamban. | |
100 μg | |
Primary | |
Canine, Chicken, Guinea Pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat | |
Antibody | |
IgG2a |
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
2D12 | |
Unconjugated | |
PLN | |
cardiac CMH18; Cardiac phospholamban; CMD1P; CMH18; phospholamban; Plb; Plm; PLN; unnamed protein product | |
Mouse | |
Protein A | |
RUO | |
100009299, 100721387, 101102067, 18821, 396378, 397421, 414755, 5350, 64672 | |
-20° C, Avoid Freeze/Thaw Cycles | |
Liquid |