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Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with GC Buffer

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Brand:  Thermo Scientific™ F532L

4960.00 SEK valid until 2022-10-31
Use promo code "16498" to get your promotional price.



Additional Details : Weight : 1.71460kg

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Product Code. 10094387

  • 6510.00 SEK / 500 reactions
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Description

Description

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user.
  • High fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Speed of the polymerase allows short extension times (15-30 s/kb)
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts

Includes:

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Specifications

Specifications

• 10 x 1.25 mL Phusion Master Mix with GC Buffer (2X) (provides 1.5 mM MgCl2 in final reaction)
• 2 x 500 μL 100% DMSO

Store at -20°C.

25 X
High
500
Phusion High-Fidelity DNA Polymerase
SuperMix or Master Mix
20 kb or Less
With GC Buffer
High-Fidelity PCR Master Mix
PCR Amplification
2X
SuperMix or Master Mix
No
Blunt
DHPLC
Fast
DNA
Phusion
500 x 50 μL Reactions
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Special Offers

Special Offers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.