Phycoerythrin, Biotin, clone: eBioPE-DLF, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-4120-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|R-phycoerythrin, R-PE, RPE, PE|
|IgG1, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.