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Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

776.00 SEK - 7090.00 SEK

Specifications

Shipping Condition Room Temperature
Column Type Proprietary Resin
Product Line Zeba™
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Products 11
Product Code Brand Description Quantity Price Quantity & Availability  
Product Code Brand Description Quantity Price Quantity & Availability  
17175762
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Thermo Scientific™
A44296S
Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL 5 Columns
776.00 SEK
Pack of 5
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17165762
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Thermo Scientific™
A44296
Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL 25 Columns
2850.00 SEK
Pack of 25
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17176368
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Thermo Scientific™
A44297
Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL 2 x 25 Columns
4545.00 SEK
Pack of 50
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17185762
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Thermo Scientific™
A44298
Zeba™ Dye and Biotin Removal Spin Columns, 2 mL 5 Columns
1078.00 SEK
Pack of 5
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16627182
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Thermo Scientific™
A44299
Zeba™ Dye and Biotin Removal Spin Columns, 2 mL 25 Columns
4305.00 SEK
Each
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17195762
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Thermo Scientific™
A44300
Zeba™ Dye and Biotin Removal Spin Columns, 5 mL 5 Columns
1266.00 SEK
Pack of 5
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Estimated Shipment: 25-10-2024
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16685344
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Thermo Scientific™
A44301
Zeba™ Dye and Biotin Removal Spin Columns, 5 mL 25 Columns
5050.00 SEK
Each
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16607425
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Thermo Scientific™
A44302
Zeba™ Dye and Biotin Removal Spin Columns, 10 mL 5 Columns
1516.00 SEK
Each
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17186368
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Thermo Scientific™
A44303
Zeba™ Dye and Biotin Removal Spin Columns, 10 mL 5 x 5 Columns
6830.00 SEK
Pack of 25
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17105772
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Thermo Scientific™
A44304
Zeba™ Dye and Biotin Removal Filter Plates, 96-well 2 Plates
4380.00 SEK
Pack of 2
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Estimated Shipment: 25-10-2024
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17196368
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Thermo Scientific™
A44305
Zeba™ Dye and Biotin Removal Filter Plates, 96-well 2 x 2 Plates
7090.00 SEK
Pack of 4
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Estimated Shipment: 01-11-2024
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Description

Description

Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 minutes
Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
Spin columns: 0.5, 2, 5, and 10 mL
96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Specifications

Specifications

Room Temperature
Proprietary Resin
Zeba™
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SDS
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Documents

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