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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Brand: Thermo Scientific™ 32132X3
Additional Details : CAS Number : 79-06-1 Weight : 0.30000kg Transport : UN number : 1993 Chem class : 3 Pack group : II
Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.Features of ECL Plus Substrate:
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Specifications
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 300 mL Kit | |
| 3000 sq. cm of membrane |
| Chemiluminescent, Fluorescent | |
| Western Blot | |
| Pierce™ | |
| Low picogram to mid-femtogram | |
| Upon receipt store at 4°C. Product shipped on ice. |
For Research Use Only. Not for use in diagnostic procedures.