missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Brand: Thermo Scientific™ 32134
1181.80 SEK valid until 2025-05-31
Use promo code "25977" to get your promotional price.
This item is not returnable.
View return policy
Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
- Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
- Higher sensitivity—detect targets down to the low-picogram level
- Longer signal duration—sustained light output for as long as 5 hours
- More imaging options—X-ray, CCD or laser-based imagers
- More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Specifications
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 25 mL Kit | |
| 2°: 1:25-200K (5-40ng/mL), 1°: 1:1K (0.05- 1μg/mL) | |
| 5 hr. | |
| Upon receipt store at 4°C. Product shipped on ice. |
| Chemiluminescence, Fluorescence | |
| Western Blot | |
| Pierce™ | |
| Horseradish Peroxidase | |
| Low picogram to mid-femtogram | |
| 250 cm2 of membrane |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce ECL Plus Western Blotting Substrate
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H351-Suspected of causing cancer.
Precautionary Statement
- P201-Obtain special instructions before use.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Ethanol
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
Promotions
-
Multiple Manufacturers
Powering Proteomics
Advance your proteomics research with essential toolsPromo Code: 25977Expires: 2025-05-31
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate > 25 mL Kit
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction