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Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Brand: Thermo Scientific™ 26128
Additional Details : Weight : 0.01000kg
Description
Thermo Scientific™ Pierce PEG4-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 4-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.
SPDP reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithio group at the opposite end. PEG4-SPDP has a 4-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent.Highlights:
Efficient amine conjugation – NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines
Reversible sulfhydryl conjugation – pyridyldithio group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond
Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules
Spacer arm – connects primary amines and sulfhydryl targets at distances up to 25.7 angstroms
Specifications:
- Chemical name (simplified): 2-pyridyldithiol-tetraoxatetradecane-N-hydroxysuccinimide
- Chemical formula: C23H33N3O9S2
- Molecular weight: 559.65
- Spacer arm: 25.7 angstroms
- Form: straw to amber colored oil
- Solubility: dissolve 2mg in 179μL DMSO or DMF to make 20mM stock for each use
Specifications
Amine-Sulfhydryl | |
PEG4-SPDP | |
Yes | |
Upon receipt store at -20°C protected from moisture. | |
Ambient | |
Chemical Labeling | |
NHS Ester, Pyridyldisulfide | |
Liquid | |
Water | |
Yes |
By Thiols | |
559.65 | |
25.7 Å | |
No | |
Pierce™ | |
Heterobifunctional | |
Medium (10 to 30 Å) | |
100 mg | |
Standard | |
Crosslinker |
For Research Use Only. Not for use in diagnostic procedures.