Thermo Scientific™ Pierce™ Rapid Antibody Isotyping Kit - Mouse

The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.

Highlights:

• Long shelf life – stable for more than 1 year at room temperature

• Single-step – add diluted antibody sample to the loading-well of cassette

• Fast – within 5 minutes a band appears that indicates the antibody isotype

• Sensitive – detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL

• Specific – assay does not crossreact with fetal bovine serum (FBS)

• Reliable – results are similar to the standard ELISA-based isotyping assays

Includes:

Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent