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Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Brand:  Thermo Scientific™ 20163

4953.58 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.01000kg

Product Code. 13434209

  • 7430.00 SEK / 20 reactions
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Description

Description

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit.


Additional Information and Online Ordering »

The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex. The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit.

Highlights:

  • Non-radioactive

    – a single biotin labels has similar detection sensitivity as radioactivity

  • Easy to use – RNA ligase and optimized reaction buffer are included

  • Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes

  • End-labeled – results in minimal disturbance of RNA secondary structure

  • Flexible

    – desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin

Includes:

  • Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents

Recommended for:

  • Northern blotting
  • EMSA
  • Enrichment of RNA binding proteins (RBP)
Specifications

Specifications

Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each
• Desthiobiotinylated Cytidine Bisphosphate, 40 μL
• T4 RNA Ligase, 40 μL
• T4 RNA Ligase Reaction buffer (10X), 100 μL
• Non-labeled RNA Control, 100 μL
• Biotinylated IRE RNA Control, 35 μL
• RNase Inhibitor, 2 x 10 μL
• DMSO, 200 μL
• PEG (30%), 300 μL
• Glycogen, 20 μL
• Nuclease-free Water, 1.5 mL

Upon receipt, store at -20°C.
Magnetic Bead
Pierce™
20 Reactions
Kit
20
RNA Labeling Kit
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For Research Use Only. Not for use in diagnostic procedures.