Thermo Scientific™ Pierce™ Streptavidin, Hydrazide-Activated

Description

Thermo Scientific™ Pierce Streptavidin and Conjugates include purified recombinant streptavidin biotin-binding protein, plus standard SA-HRP, SA-AP and fluorescent conjugates for biotin-based assays.

These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.

Highlights:

• Recombinant streptavidin – high-quality protein, 53kDa, pI equals 6.8 to 7.5

• Pure protein and enzyme conjugates – choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection

• Fluorophore conjugates – standard fluorescent forms (fluorescein, rhodamine, etc.) for traditional immunofluorescence techniques

• Activated preparations – choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule