Thermo Scientific Pierce Strong Cation and Anion Ion Exchange Columns use adsorbent chromatographic matrices to fractionate proteins based on charge differences. The adsorbent membranes and column structures are optimized for rapid sample processing by centrifugation. The membrane-based adsorbents have a highly porous structure (pores larger than 3000nm), providing proteins unhindered access to the charged affinity ligands. These features provide for high efficiencies and flow rates when fractionating large biomolecules with small diffusivities. Columns are available in mini (0.5mL microcentrifuge) and maxi (20mL centrifuge) sizes in both anionic and cationic formats.
Pierce Ion Exchange Spin Columns replace time-consuming, tedious and expensive chromatographic methods for many protein applications. The centrifuge-purification format based on membrane adsorbents allows convenient high-yield processing of multiple samples in less than 20 minutes. The purified protein is compatible with downstream applications such as sample fractionation for 1D and 2D SDS-PAGE, X-ray crystallization and NMR spectroscopy.
Fast and simple – membrane-based spin format eliminates column packing required with traditional resin methods
Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel
Durable and robust – spin columns and integrated adsorbent membranes do not crack or fail when run dry
Small bed volume – adsorbent membrane has a very small effective bed volume, which minimizes sample loss and dilution effects with small-volume samples
Anion and cation formats – choose either negatively charged or positively charged ion exchange columns
Two column sizes – choose either Mini size (4mg, 0.5mL capacity) or Maxi size (80mg, 20mL capacity) columns
Ready-to-use columns and two collection tubes per column
Pre-fractionation of proteins in lysate; Scouting purification conditions for new protein preparation protocols; Removal of endotoxins from monoclonal antibodies; Polishing His-tagged proteins after metal chelate chromatography; Purification of antibodies from serum, ascites or tissue culture supernatant; Removal of detergent from protein solutions; Sample preparation before 1D or 2D PAGE; Purification of phosphopeptides before MS analysis.