missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ Thiophilic Adsorption Kit

Preferentially bind and purify immunoglobulins from various sources including mouse, rat, rabbit, goat and human with this non-proteinaceous immunosorbent resin.

Brand:  Thermo Scientific™ 44916

4226.87 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.10000kg Transport : UN number : 2811 Chem class : 6.1 Pack group : III

Product Code. 10097143

  • 6340.00 SEK / 1 set
Estimated Shipment: 16-04-2024
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Thermo Scientific™ Pierce Thiophilic Adsorbent is a non-proteinaceous immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human.

Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity. The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers.

Highlights:

  • High capacity – gently bind and purify approx. 20mg polyclonal antibody per milliliter of adsorbent resin

  • Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY

  • Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps

  • Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody

Includes:

Kit contains four Thiophilic Adsorbent Columns (3mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories

Recommended for:

Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications

Specifications

Specifications

Adsorption Kit
Agarose Resin, Affinity
Sulfone Thioether
Pierce Thiophilic Adsorption Kit
4 Columns
Pierce™
Gravity Flow Column
IgG Antibody
Sufficient For: Purifying up to 60 mg immunoglobulin per column
• Thiophilic Adsorbent Columns, 3 mL, 4 columns
• Binding Buffer, 1 L
• Elution Buffer, 1 L
• Column Storage Buffer (2X), 100 mL
• Guanidine-HCl Crystals, 230 g
• Column Accessories

Store at 4°C.
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.