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Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Brand:  Thermo Scientific™ 34021

Additional Details : Weight : 1.81440kg


Product Code. 10076433

  • 2240.00 SEK / 400mL

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Description

Description

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.

Highlights:

  • Ready-to-use solutions
  • No hydrogen peroxide required
  • No filtering required
  • Noncarcinogenic
  • Various sensitivities to suit any assay

1-Step TMB Substrates

  • Ready-to-use, one-component substrates
  • Do not require hydrogen peroxide
  • Noncarcinogenic
  • Do not contain DMF or DMSO
  • Available in three formulations that vary by sensitivity

TMB Substrate Kit

  • More sensitive than OPD in endpoint ELISAs
  • Easy to use
  • Provides results in seconds

Includes:

TMB Substrate Kit (PI-34021): Peroxidase substrate, 200mL; Peroxide solution, 200mL

Specifications

Specifications

Substrate Solution, 200mL Stable Peroxide Solution

Pierce™
400 mL Kit
HRP (Horseradish Peroxidase) Substrate
Colorimetric
ELISA Substrate
Chemical Substrate
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Safety and Handling

Safety and Handling

Product Identifier
  • Pierce TMB Substrate Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • Specific target organ toxicity after single exposure Category 1

Hazard Statement
  • H302-Harmful if swallowed.
  • H312-Harmful in contact with skin.
  • H332-Harmful if inhaled.
  • H370-Causes damage to organs.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P405-Store locked up.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : methanol, citric acid

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