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PIK3C3 (hVPS34) Recombinant Human Protein

PIK3C3 (hVPS34) Recombinant Human Protein

Brand:  Invitrogen PR8987B

Additional Details : Weight : 0.01000kg

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Product Code. 15448703

  • 20540.00 SEK / 100µg


Estimated Shipment date
from Supplier 04-01-2018
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking.
Specifications

Specifications

NP_002638.2
Protein assays and analysis
Frozen
PIK3C3 (hVPS34) Recombinant Human Protein
100μg
For research use only. Not for use in diagnostic procedures
PIK3C3 (hVPS34)
PIK3C3 (hVPS34) was diluted in assay buffer and the activity determined in a 10μL Adapta™ assay (PV5099) with 10μM ATP and 100μM PI:PS lipid kinase substrate (PV5122) at room temperature for 1 hour. The reaction was stopped with a 5μL addition of EDTA and detection reagents. The final concentrations are 10mM EDTA, 2nM Adapta™ Eu-anti-ADP antibody and 10nM Alexa Fluor™ 647 ADP Tracer. The plate was read after a 45 min. room temperature incubation
Baculovirus
PIK3C3 (hVPS34) Recombinant Human Protein
Human
0.6mg/mL
Frozen
129kDa
90% by SDS-PAGE
–80°C
1 Each
PIK3C3 (hVPS34) Recombinant Human Protein
Kinase assay
PI3
23.4nmoles of ADP formed per minute per mg of total protein using the Adapta™ assay (PV5099). An ADP/ATP standard curve is used to calculate percent conversion of ATP to ADP. PIK3C3 (hVPS34) activity determined at a final protein concentration of 625ng/mL
GST Tag
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