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PLK4 Recombinant Human Protein

PLK4 Recombinant Human Protein

Brand:  Invitrogen PV6394

Additional Details : Weight : 0.01000kg

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Product Code. 15449023

  • 2975.00 SEK / 10µg


Estimated Shipment date
from Supplier 04-01-2018
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells.The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development. Recombinant human PLK4 (1-836) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance
  • Multiple pack sizes available for convenient use

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  • Specifications

    Specifications

    NP_055079.3
    Pharma and biopharma, protein assays and analysis, target and lead identification and validation
    PLK4 Recombinant Human Protein
    10μg
    For research use only. Not for use in diagnostic procedures
    SAK, STK18
    PLK4 was pre-diluted in enzyme dilution buffer and assayed in 5mM MOPS (pH 7.2), 2.5mM β-glycerol-phosphate, 5mM MgCl2, 0.4mM EDTA, 1mM EGTA, 0.05mM DTT, with 50μM ATP, trace [33P]-γ-ATP and 200μg/mL myelin basic protein (MBP) for 15 min.s at 30°
    Amino acids 1-836
    6.4nmoles of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein at 30°C
    GST Tag
    0.05mg/mL
    120.9kDa
    >70% by SDS-PAGE
    –80°C
    1 Each
    PLK4 Recombinant Human Protein
    Kinase assay
    PLK4 Recombinant Human Protein
    Human
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