Poly-L-Lysine Hydrobromide, MP Biomedicals™

Poly-lysine binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interactions to promote cell adhesion. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

• When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding
• When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding

Other applications of Poly-L-lysine include:

• Conjugation to methotrexate for increased drug transport
• Microencapsulation of islets
• Use in a simple reproducible procedure for chromosomal preparations from a variety of tissues
• Immobilation of living cells in biocompatible semipermeable microcapsules
• In the preparation of polycationic beads
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake