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Thermo Scientific™ Polymyxin Egg Yolk Mannitol Bromothymol Blue Agar (PEMBA)
Isolate and enumerate Bacillus cereus with selective and diagnostic Polymyxin Egg Yolk Mannitol Bromothymol Blue Agar.
Brand: Thermo Scientific™ PO5048A
203.41 SEK valid until 2025-01-31
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Description
Isolate and enumerate Bacillus cereus from food samples with selective and diagnostic Thermo Scientific™ Oxoid™ Polymyxin Egg Yolk Mannitol Bromothymol Blue Agar (PEMBA). The ISO11133:2014 tested PEMBA medium is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants. The medium is also sufficiently diagnostic that colonies of Bacillus cereus are readily identified and confirmed by microscopic examination1.
The role of Bacillus cereus in food poisoning, particularly from the consumption of contaminated rice, is now well documented2,3,4.
In the formulation of PEMBA Agar, a peptone level of 0.1% and the addition of sodium pyruvate improve egg yolk precipitation and enhance sporulation. Bromothymol blue is added as a pH indicator to detect mannitol utilization. The medium is made selective by addition of Bacillus Cereus Selective Supplement, which gives a final concentration of 100 IU of polymyxin B per mL of medium. Polymyxin B is a selective agent for the isolation of Bacillus cereus5.
The typical colonies of Bacillus cereus are crenated, about 5mm in diameter, and have a distinctive turquoise to peacock-blue color surrounded by a good egg yolk precipitate of the same color. These features distinguish Bacillus cereus from other Bacillus species except Bacillus thuringiensis. Other egg yolk-reacting organisms which can grow on the medium, including Staphylococcus aureus, Serratia marcescens, and Proteus vulgaris are distinguished from Bacillus cereus by colony form and color. These organisms also produce an egg yolk-clearing reaction in contrast to egg yolk precipitate produced by Bacillus cereus.
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Specifications
| Sulphur yellow, transparent | |
| Polymyxin Egg Yolk Mannitol Bromothymol Blue Agar (PEMBA) | |
| Germany | |
| 17 mL | |
| Single Layer |
| 2° to 12°C, in the dark | |
| 90mm Monoplate | |
| Agar | |
| 17g ± 5% | |
| 10/Pk. |