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PRKCE (PKC epsilon) Recombinant Human Protein

PRKCE (PKC epsilon) Recombinant Human Protein

Brand:  Invitrogen PR1473D

Additional Details : Weight : 0.01000kg

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Product Code. 15468373

  • 12870.00 SEK / 100µg


Estimated Shipment date
from Supplier 08-12-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

The PKC family of serine⁄threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_005391.1
Protein assays and analysis
Frozen
PRKCE (PKC epsilon) Recombinant Human Protein
100μg
For research use only. Not for use in diagnostic procedures
PRKCE (PKC epsilon)
PRKCE (PKC epsilon) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.5), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
Baculovirus
PRKCE (PKC epsilon) Recombinant Human Protein
Human
0.48mg/mL
Frozen
83.7kDa
90% by SDS-PAGE
–80°C
1 Each
PRKCE (PKC epsilon) Recombinant Human Protein
Kinase assay
PKC
5,330nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25 to 2μg/mL
None
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