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Invitrogen™ Progesterone Competitive ELISA Kit
Competitive ELISA
Brand: Invitrogen™ EIAP4C21
3866.86 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Additional Details : Weight : 0.33820kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Progesterone ELISA quantitates progesterone in urine,dried fecal extracts or cell culture medium.
Principle of the method
The Progesterone Competitive ELISA research-use-only kit is designed to quantitatively measure Progesterone present in extracted dried fecal samples,urine,and tissue culture media samples. A progesterone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A progesterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to progesterone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound progesterone-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Specifications
50-3200 pg/mL | |
Progesterone Competitive ELISA Kit | |
HRP | |
ELISA Kit | |
Fecal Extract, Supernatant, Urine | |
Human | |
Colorimetric Microplate Reader | |
3.9% | |
HRP | |
RUO | |
Fecal Extract,50 μL; Supernatant, 50 μL; Urine,12.5 μL | |
Chemical | |
2 hr. 30 min. |
47.9 pg/mL | |
Multiplex assay on the Luminex Platform | |
Fluorescence | |
Steroid hormone | |
96 assays | |
ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
5.7% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Reproductive Biology | |
-20°C | |
1 hr. 20 min. |