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Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns
Beskrivning
Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.
- Retention control by imidazole concentration or pH gradient
- Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
- Effective under native or denaturing HPLC conditions
- Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
- Shipped free from any metal and ready to be charged with the metal of your choice.
- Reusable columns with metal tailored specificity
- Resolve target proteins using a single column in a high resolution gradient run
- High purity separations of metal-binding proteins
Specifikationer
Specifikationer
| Kolumnformat | Analytical Column |
| Diameter (metrisk) | 2 mm |
| Flödeshastighet | 1 mL/min |
| För användning med | UltiMate 3000 HPLC System |
| Längd (metrisk) | 50 mm |
| Max. Tryck | 3000 psi (207 bar) |
| Mobilfaskompatibilitet | pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl |
| Partikelstorlek | 10 μm |
| pH | 2 to 12 |
| Porstorlek | Nonporous |
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