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Merck Millipore Proteinase K

From Tritirachium album

Brand:  Merck Millipore 1245680500

Additional Details : CAS Number : 39450-01-6 Weight : 0.00050kg


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Product Code. 10036570

  • 4800.00 SEK / 500mg
Estimated Shipment: 07-03-2023
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Description

Description

  • Chromatographically purified lyophilized 30 m Anson-U/mg
  • For biochemistry and molecular biology EC 3.4.21.14
  • Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
  • Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
  • Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
  • Determination of enzyme localization on membranes
  • Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
  • Digestion of proteins from brain tissue samples for prions in Transmissible
  • Spongiform Encephalopathies (TSE) research.
  • Proteinase K is a stable and highly reactive serine protease.
  • Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224).
  • It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature.
  • In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
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Safety and Handling

Safety and Handling

Product Identifier
  • PROTEINASE K CHROMATOgrAPHICALLY PURIFIED, 500mg

Signal Word
  • Danger

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash skin thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.

Supplemental information
  • MIXTURE LIST-Contains : proteinase k

SDS
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