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Macherey-Nagel Bioanalysis™ Protino™ Ni-NTA Agarose

The method of choice for His-tag protein purification.

Brand:  Macherey-Nagel Bioanalysis™ 745400.500

Additional Details : Weight : 1.25000kg

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Product Code. 11952192

  • 39662.00 SEK / 500mL

Estimated Shipment: 13-08-2021
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  • Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
  • Proteins from any expression system can be purified under native or denaturing conditions.
  • Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
  • Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
  • The resin is precharged with Ni2+ ions and therefore ready to use.
  • Technology: Immobilized metal ion affinity chromatography
  • Batch binding, gravity flow column chromatography, MPLC / FPLC applications
  • Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
  • Bead size: 45 to 165 μm


Purification of His-tag proteins
Aqueous suspension
500 mL
4 to 8°C
< 50 mg/mL settled agarose
NTA (nitrilotriacetic acid)
Protein solution
Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3
  • Skin sensitiser Category 1

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H317-May cause an allergic skin reaction.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information
  • MIXTURE LIST-Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in