missing translation for 'onlineSavingsMsg'
Learn More

PU.1, clone: phpu13, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9819-82

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15258187

  • 1806.00 SEK / 100µg


EU Stock 5
Add to basket

Description

Description

Description: The monoclonal phpu13 recognizes human and mouse PU.1, a member of the ets family of transcription factors. This 42kDa protein was originally identified as Spi-1, a proto-oncogene, for its role in Friend virus-induced murine erythroleukemia. Expression of PU.1 plays a critical role in lymphocyte and myeloid development. Knock-out mice are neonatal lethal and lack myeloid, T and B cells. In addition erythroid development is also altered. Protein expression levels help guarantee proper development; higher levels in myeloid cells compared to lymphocytes. PU.1 controls transcription of several proteins including CD11b, CD18, Gata2, IRF4, CD20, CD10, and CD79.Disruption of PU.1 expression may play a role in several specific leukemias; such as B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin.Applications Reported: This phpu13 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and immunocytochemistry.Applications Tested: This phpu13 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using IHC Antigen Retrieval Solution - Low pH (10X) (cat. 00-4955). This can be used at less than or equal to 10 ug/mL. It is recommeded that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

PU.1
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P17433, P17947
31kDa transforming protein, 31kDa-transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1, transcription factor PU.1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
ImmunocytochemistryþImmunofluorescenceþImmunohistochemistry (Paraffin)
phpu13
Unconjugated
SPI1
Liquid
Spi1
IgG1, kappa, kappa
100μg
Store at 2-8°C.
Primary
20375, 6688
Videos

For Research Use Only.