Thermo Scientific™ qualyfast™ Legionella PCR and DNA Extraction Kits

Minimizes false negatives

• Free DNA Inactivator removes DNA from non-viable sources commonly found in environmental water samples.
• DNA Extraction Kits remove environmental contaminants minimizing potential inhibition effects.
• PCR Assays with simultaneous co-amplification of an Internal Application Control allowing determination of potential inhibition effects.

Increases accuracy

• Quantification Standards enable PCR detection of <i>Legionella</i> species to be quantified within the 25-25,000 Genomic Unit range.
• Increases throughput: Multiplexed PCR of the characteristic DNA sequences for species within the Legionella genus and the specific species L. pneumophilia.

Easy-to-use

• PCR Assay kits contain strips with eight reaction tubes containing lyophilized PCR reagents, DNA free solution and pre-dosed positive-control tubes.
• Quantification Standards are pre-aliquoted and lyophilized in individual tubes.
• DNA Extraction Kits contain all the consumables required to extract DNA from environmental water samples.

Optimized workflow

• Complete end-to-end workflow covering sample preparation using DNA Extraction Kits and Free DNA Inactivator, detection using multiplex Legionella PCR Assay and evaluation of results using Quantification Standards
• ISO compliant: workflow is compliant with the protocol, experimental design and calculation methods described by ISO/TS 12869:2012

The qualyfast® Legionella PCR Assay contains:

•  Pre-dosed and lyophilized PCR reagents in strips of 8 reaction tubes
• A strip of 8 reaction tubes containing positive controls
• DNA-free solution

The qualyfast® DNA Extraction Kit I is designed for use with clear water samples, as acquired from water distribution systems, drinking water and other sources that contain minimal suspended particles. The kit contains:

• Single-use, sterile membrane filters
• Lysis buffer for enhanced isolation of microorganisms from filter membranes
• Lysis enzyme for DNA extraction

The qualyfast® DNA Extraction Kit II is designed for use with dirty water, biofilm and sediment samples. The kit contains:

• Single-use, sterile membrane filters
• Pre-lysis buffer
• Lysis solution & bead beating lysis tubes
• Spin filter
• Post-lysis filtration columns and reagents
• All collection tubes required for sample handling during extraction

The qualyfast® Free DNA Inactivator Kit contains:

• DNA inactivation enzyme (lyophilized)
• DNA inactivation buffers

The qualyfast® Legionella Quantification Standards Kit contains:

• Four different quantified standards, each packaged in strips of 8 reaction tubes, enabling 8 analysis sessions
• DNA-free solution for reconstitution of lyophilized reagents and negative-control

The qualyfast® Legionella workflow is compatible with the Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR System or Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System.