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Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™, Invitrogen™

Rat Isotype Control

3125.00 SEK

Specifications

Antigen Rat IgG2b kappa
Clone eB149/10H5
Applications Control, Flow Cytometry
Conjugate Super Bright 600
Form Liquid
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Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
16351484
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Affymetrix eBioscience
63-4031-82
100 μg
3125.00 SEK
100µg
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Estimated Shipment: 16-04-2024
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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Rat IgG2b kappa Control antibody specifically detects Rat IgG2b kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.
Specifications

Specifications

Rat IgG2b kappa
Control, Flow Cytometry
Liquid
RUO
PBS with BSA and 0.09% sodium azide; pH 7.2
Isotype Control (Primary)
eB149/10H5
Super Bright 600
Rat
Not Applicable
IgG2b κ
Affinity chromatography
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