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R&D Systems™ Recombinant F. meningosepticum PNGase F Protein

Produktkod. 18757267
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18757267 100 μg
18735817 20 μg
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Produktkod. 18757267 Leverantör R&D Systems™ Leverantörsnummer 9109GH100
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Beräknad leverans: 15-10-2026
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Descripción

Effective removal of N-linked oligosaccharides from glycoproteins. Glycerol-free, suitable for downstream MS and HPLC.

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.
TRUSTED_SUSTAINABILITY

Especificaciones

Tillträdesnummer P21163
För användning med (applikation) Enzyme Activity
Formulering Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molekylvikt (g/mol) M.W. (Observed): 34 kDa, reducing conditions; M.W. (theoretical): 36 kDa
Kvantitet 100 μg
Källa E. coli-derived f. meningosepticum PNGase F protein Ala41-Asn354 with N-terminal Met and 6-His tag
Förvaringskrav Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxinkoncentration <1.0 EU / 1 μg of the protein by the LAL method.
Vanligt namn PNGase F
Biologisk aktivitet Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.
Konjugera Unconjugated
Art F. meningosepticum
Rekombinant Recombinant
Uttryckssystem E. coli-derived Ala41-Asn354 with N-terminal Met and 6-His tag
Produktlinje R&D Systems
Renhet eller kvalitetsklass >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PNGase F
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