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R&D Systems™ Recombinant Human Thrombopoietin/TPO Protein, Animal-Free
Beskrivning
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.050-0.500 ng/mL. The specific activity of Recombinant Human Thrombopoietin is >1 x 107 units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).
Specifikationer
Specifikationer
| Tillträdesnummer | NP_000451.1 |
| För användning med (applikation) | Bioactivity |
| Formulering | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose. |
| Gen-ID (Entrez) | 7066 |
| Molekylvikt (g/mol) | M.W.-theoretical: 19 kDa, M.W.-observed: 18-19 kDa, under reducing conditions. |
| Kvantitet | 1 mg |
| Källa | E. coli-derived human Thrombopoietin/Tpo protein Ser22-Leu195 Produced using non-animal reagents in an animal-free laboratory. |
| Förvaringskrav | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Endotoxinkoncentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF |
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