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R&D Systems™ Recombinant Mouse B7-H4 Fc Chimera Protein
Beskrivning
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and Recombinant Mouse B7-H4 Fc Chimera. The ED50 for this effect is 0.5-3 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
Specifikationer
Specifikationer
| Tillträdesnummer | AAP37284 |
| Karakterisering | Bioactivity |
| För användning med (applikation) | Bioactivity |
| Formulering | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Gen-ID (Entrez) | 79679 |
| Molekylvikt (g/mol) | M.W. (Observed): 75-85 kDa, reducing conditions; M.W. (theoretical): 52.4 kDa (monomer) |
| Kvantitet | 1 mg |
| Källa | Mouse myeloma cell line, NS0-derived mouse B7-H4 protein Mouse B7-H4 (Phe29-Pro258) (N-terminus) Accession # AAP37284 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus) |
| Förvaringskrav | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Endotoxinkoncentration | <0.01 EU / 1 μg of the protein by the LAL method. |
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Produkttitel
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