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Novus Biologicals™ Recombinant PreScission Protease Protein
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Quantity:
100 IU
250 IU
Unit Size:
Each
Description
The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein.

Specifications
Specifications
For Use With (Application) | SDS-PAGE, Enzyme Activity |
Formulation | 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. |
Molecular Weight (g/mol) | MolecularWeight-theroretical: 43 kDa |
Quantity | 250 IU |
Storage Requirements | Store at -20°C. Avoid freeze/thaw cycles. |
Gene Alias | 3 C protease, Picornain 3 C, PSP |
Inhibitor Properties | During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. It works most effective at 4 C but can digest substrates (at room temperature. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20 or NonidetTM P40 to a concentration of 0.01 %. Concentrations of these detergents up to 1 % do not inhibit PreScission Protease.) |
Purity or Quality Grade | Peptide affinity purified |
Protein | PreScission Protease |
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