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Novus Biologicals™ Recombinant PreScission Protease Protein Product Code.: 18602248

Novus Biologicals™ Recombinant PreScission Protease Protein

Product Code. p-200083673 Shop All Bio Techne Products
250 IU, Each
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Quantity:
100 IU
250 IU
Unit Size:
Each
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Product Code. 18602248

Brand: Novus Biologicals™ NBP261442250IU

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This item is not returnable. View return policy

Recombinant Protein

The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein.
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) SDS-PAGE, Enzyme Activity
Formulation 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
Molecular Weight (g/mol) MolecularWeight-theroretical: 43 kDa
Quantity 250 IU
Storage Requirements Store at -20°C. Avoid freeze/thaw cycles.
Gene Alias 3 C protease, Picornain 3 C, PSP
Inhibitor Properties During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. It works most effective at 4 C but can digest substrates (at room temperature. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20 or NonidetTM P40 to a concentration of 0.01 %. Concentrations of these detergents up to 1 % do not inhibit PreScission Protease.)
Purity or Quality Grade Peptide affinity purified
Protein PreScission Protease
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