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Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis.
Brand: Ambion™ A1083708
Additional Details : Weight : 0.10000kg
Description
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.
The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology
Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).
Specifications
Each kit contains: |
|
| Total RNA | |
| High-throughput Compatible | |
| 8 Preps | |
| 1 Each | |
| Wet Ice | |
| Transcriptome RNA | |
| 1 μg |
| Magnetic Bead | |
| Transcriptome RNA | |
| Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction | |
| RiboMinus™ | |
| Transcriptome Sequencing | |
| 2-10 μg Total RNA | |
| RiboMinus |
For Research Use Only. Not for use in diagnostic procedures.