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Merck Millipore RIPA Lysis Buffer, 10X

For use in immunoprecipitation and Western blotting. RIPA Lysis Buffer, 10X is a concentrated lysis buffer that has been filtered through a 0.2μm membrane and packaged aseptically.

Brand:  Merck Millipore 20-188

Additional Details : Weight : 0.11700kg


Product Code. 10014424

  • 1791.27 SEK / Pack of 10
Estimated Shipment: 20-12-2021

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Description

Description

Protein extraction and purification represent the first of many challenges in obtaining a quality lysate or purified protein sample that delivers publication-quality Western blot results. Merck quality reagents unite superior performance with speed to reduce exposure of proteins to unfavorable conditions, leading to more stable, intact proteins for downstream analysis.

  • Use: For use in mammalian cell lysis
  • Dilute one part 10X RIPA Lysis Buffer with nine parts sterile, distilled water
  • Stir mixture and refrigerate at least two hours prior to use
  • Immediately prior to use, add the protease and phosphatase inhibitors
  • Filtered through 0.2μm membrane, packaged aseptically
Specifications

Specifications

Concentrated lysis buffer
100mL
Immunoprecipitation, Western blot
10X
0.5M Tris-HCl, pH 7.4, 1.5M NaCl, 2.5% deoxycholic acid, 10% NP-40, 10mM EDTA.
Liquid
Clear, colorless
Sterile
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Safety and Handling

Safety and Handling

Product Identifier
  • RIPA Lysis Buffer, 10X

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash skin thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information
  • MIXTURE LIST-Contains : hydroxy-poly(oxy-1,2-ethanediyl) branched, deoxycholic acid

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